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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
20/12/2018 |
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Data da última atualização: |
27/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VARELI, C. S.; PIZZUTTI, I. R.; GEBLER, L.; CARDOSO, C. D.; GAI, D. S. H.; FONTANA, M. E. Z. |
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Afiliação: |
Catiucia S. Vareli, UFSM – Federal University of Santa Maria, Chemistry Department, Center of Research and Analysis of Residues and Contaminants (CEPARC), Campus, 97105-900 Santa Maria, RS, Brazil; Ionara R. Pizzutti, a UFSM – Federal University of Santa Maria, Chemistry Department, Center of Research and Analysis of Residues and Contaminants (CEPARC), Campus, 97105-900 Santa Maria, RS, Brazil; LUCIANO GEBLER, CNPUV; Carmem D. Cardoso, a UFSM – Federal University of Santa Maria, Chemistry Department, Center of Research and Analysis of Residues and Contaminants (CEPARC), Campus, 97105-900 Santa Maria, RS, Brazil; Daniela S.H. Gai, a UFSM – Federal University of Santa Maria, Chemistry Department, Center of Research and Analysis of Residues and Contaminants (CEPARC), Campus, 97105-900 Santa Maria, RS, Brazil; Marlos E.Z. Fontana, a UFSM – Federal University of Santa Maria, Chemistry Department, Center of Research and Analysis of Residues and Contaminants (CEPARC), Campus, 97105-900 Santa Maria, RS, Brazil. |
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Título: |
Analytical method validation to evaluate dithiocarbamates degradation in biobeds in South of Brazil. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Talanta, v. 184, p. 202-209, 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
In order to evaluate the efficiency of biobeds on DTC degradation, the aim of this study was to apply, optimize and validate a method to determine dithiocarbamate (mancozeb) in biobeds using gas chromatography-tandem mass spectrometry (GC-MS). The DTC pesticide mancozeb was hydrolysed in a tin (II) chloride solution at 1.5% in HCl (4 mol L−1 ), during 1 h in a water bath at 80 °C, and the CS2 formed was extracted in isooctane. After cooling, 1 mL of the organic layer was transferred to an auto sampler vial and analyzed by GC-MS. A complete validation study was performed and the following parameters were assessed: linearity of the analytical curve (r2 ), estimated method and instrument limits of detection and limits of quantification (LODm, LODi, LOQm and LOQi, respectively), accuracy (recovery%), precision (RSD%) and matrix effects. Recovery experiments were carried out with a standard spiking solution of the DTC pesticide thiram. Blank biobed (biomixture) samples were spiked at the three levels corresponding to the CS2 concentrations of 1, 3 and 5 mg kg−1 , with seven replicates each (n = 7). The method presented satisfactory accuracy, with recoveries within the range of 89?96% and RSD ≤ 11%. The analytical curves were linear in the concentration range of 0.05?10 µg CS2 mL−1 (r2 > 0.9946). LODm and LOQm were 0.1 and 0.5 mg CS2 kg−1 , respectively, and the calculated matrix effects were not significant (≤ 20%). The validated method was applied to 80 samples (biomixture), from sixteen different biobeds (collected at five sampling times) during fourteen months. Ten percent of samples presented CS2 concentration below the LOD (0.1 mg CS2 kg−1 ) and 49% of them showed results below the LOQ (0.5 mg CS2 kg−1 ), which demonstrates the biobeds capability to degrade DTC. MenosIn order to evaluate the efficiency of biobeds on DTC degradation, the aim of this study was to apply, optimize and validate a method to determine dithiocarbamate (mancozeb) in biobeds using gas chromatography-tandem mass spectrometry (GC-MS). The DTC pesticide mancozeb was hydrolysed in a tin (II) chloride solution at 1.5% in HCl (4 mol L−1 ), during 1 h in a water bath at 80 °C, and the CS2 formed was extracted in isooctane. After cooling, 1 mL of the organic layer was transferred to an auto sampler vial and analyzed by GC-MS. A complete validation study was performed and the following parameters were assessed: linearity of the analytical curve (r2 ), estimated method and instrument limits of detection and limits of quantification (LODm, LODi, LOQm and LOQi, respectively), accuracy (recovery%), precision (RSD%) and matrix effects. Recovery experiments were carried out with a standard spiking solution of the DTC pesticide thiram. Blank biobed (biomixture) samples were spiked at the three levels corresponding to the CS2 concentrations of 1, 3 and 5 mg kg−1 , with seven replicates each (n = 7). The method presented satisfactory accuracy, with recoveries within the range of 89?96% and RSD ≤ 11%. The analytical curves were linear in the concentration range of 0.05?10 µg CS2 mL−1 (r2 > 0.9946). LODm and LOQm were 0.1 and 0.5 mg CS2 kg−1 , respectively, and the calculated matrix effects were not significant (≤ 20%). The validated method was app... Mostrar Tudo |
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Palavras-Chave: |
Biobed; Biomixture; Dithiocarbamates; GC-MS. |
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Thesaurus Nal: |
Biodegradation; Degradation. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/189181/1/Analytical-method-validation-to-evaluat...radation-in-biobeds-in-South-of-Brazil.pdf
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Marc: |
LEADER 02543naa a2200253 a 4500
001 2102339
005 2019-04-27
008 2018 bl uuuu u00u1 u #d
100 1 $aVARELI, C. S.
245 $aAnalytical method validation to evaluate dithiocarbamates degradation in biobeds in South of Brazil.$h[electronic resource]
260 $c2018
520 $aIn order to evaluate the efficiency of biobeds on DTC degradation, the aim of this study was to apply, optimize and validate a method to determine dithiocarbamate (mancozeb) in biobeds using gas chromatography-tandem mass spectrometry (GC-MS). The DTC pesticide mancozeb was hydrolysed in a tin (II) chloride solution at 1.5% in HCl (4 mol L−1 ), during 1 h in a water bath at 80 °C, and the CS2 formed was extracted in isooctane. After cooling, 1 mL of the organic layer was transferred to an auto sampler vial and analyzed by GC-MS. A complete validation study was performed and the following parameters were assessed: linearity of the analytical curve (r2 ), estimated method and instrument limits of detection and limits of quantification (LODm, LODi, LOQm and LOQi, respectively), accuracy (recovery%), precision (RSD%) and matrix effects. Recovery experiments were carried out with a standard spiking solution of the DTC pesticide thiram. Blank biobed (biomixture) samples were spiked at the three levels corresponding to the CS2 concentrations of 1, 3 and 5 mg kg−1 , with seven replicates each (n = 7). The method presented satisfactory accuracy, with recoveries within the range of 89?96% and RSD ≤ 11%. The analytical curves were linear in the concentration range of 0.05?10 µg CS2 mL−1 (r2 > 0.9946). LODm and LOQm were 0.1 and 0.5 mg CS2 kg−1 , respectively, and the calculated matrix effects were not significant (≤ 20%). The validated method was applied to 80 samples (biomixture), from sixteen different biobeds (collected at five sampling times) during fourteen months. Ten percent of samples presented CS2 concentration below the LOD (0.1 mg CS2 kg−1 ) and 49% of them showed results below the LOQ (0.5 mg CS2 kg−1 ), which demonstrates the biobeds capability to degrade DTC.
650 $aBiodegradation
650 $aDegradation
653 $aBiobed
653 $aBiomixture
653 $aDithiocarbamates
653 $aGC-MS
700 1 $aPIZZUTTI, I. R.
700 1 $aGEBLER, L.
700 1 $aCARDOSO, C. D.
700 1 $aGAI, D. S. H.
700 1 $aFONTANA, M. E. Z.
773 $tTalanta$gv. 184, p. 202-209, 2018.
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Embrapa Uva e Vinho (CNPUV) |
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| 1. |  | LIMA, G. C. de; SOARES FILHO, C.; TEIXEIRA FILHO, M.; HUNGRIA, M.; SA, G. de; MORETTI, M.; KNOLL, M. Chlorophyll content and accumulation of mass of Panicum maximum cv. BRS Zuri inoculated with bacteria promoting growth. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 55.; CONGRESSO BRASILEIRO DE ZOOTECNIA, 28., 2018, Goiânia. Construindo saberes, formando pessoas e transformando a produção animal: abstracts... Goiânia: Sociedade Brasileira de Zootecnia, 2018. trab. 1821.| Tipo: Resumo em Anais de Congresso |
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